Related Experiment Video
Updated: Aug 14, 2026
07:31
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
Published on: February 17, 2023
eVLP compound delivery breaks the prime editing efficiency ceiling
Margaret R Wang1, Francisco J Sánchez-Rivera2
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
Cell Genomics
|August 12, 2026
Summary
Prime editing efficiently resolves gene variant function using a novel repeated dosing strategy. Engineered virus-like particles (PRIME-VLP) enhance gene editing efficiency and screening performance.
Area of Science:
- Molecular Biology
- Gene Editing Technologies
- Biotechnology
Background:
- Prime editing offers a powerful tool for understanding gene variant function at scale.
- Efficient and reproducible delivery of prime editing machinery remains a significant challenge.
- Current delivery methods may limit the throughput and effectiveness of gene editing applications.
Purpose of the Study:
- To identify rate-limiting factors in prime editor delivery.
- To develop an improved delivery strategy for enhanced prime editing efficiency.
- To optimize gene editing performance for large-scale functional studies.
Main Methods:
- Investigated the impact of editor delivery timing on prime editing outcomes.
- Developed and utilized engineered virus-like particles (PRIME-VLP) for editor delivery.
- Implemented a repeated dosing strategy with PRIME-VLP.
- Assessed editing efficiency and screening performance using the new strategy.
Main Results:
- The timing of editor delivery was identified as a critical rate-limiting step.
- The PRIME-VLP strategy significantly improved prime editing efficiency compared to standard methods.
- Repeated dosing with PRIME-VLP enhanced the performance of gene editing screens.
- Demonstrated reproducible delivery and editing with the engineered virus-like particles.
Conclusions:
- Optimizing editor delivery timing is crucial for scalable gene variant function studies.
- PRIME-VLP represents a promising advancement in gene editing delivery systems.
- The repeated dosing strategy with engineered virus-like particles enhances the utility of prime editing for research and therapeutic applications.
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