Related Experiment Video
Updated: May 25, 2025
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
Published on: April 5, 2019
Quantitative and qualitative evaluation of anti-pneumococcal specific antibodies in children with recurrent acute
Masamitsu Kono1, Eisuke Kuroda2,3, Takuro Iyo1
1Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan.
Background:
Pneumococcal conjugated vaccine (PCV) has contributed to a dramatic reduction in invasive pneumococcal diseases. However, its impact upon nasopharyngeal carriage and upper respiratory tract infections is unclear.
Aims/Objectives:
This study was aimed to evaluate the humoral immunity against Streptococcus pneumoniae in children with recurrent acute otitis media (rAOM) in the post PCV era.
Materials And Methods:
We evaluated the humoral immunity against eight representative serotypes included in PCV13 (3, 6 A, 6B, 9 V, 14, 18 C, 19 F, and 23 F) among children with rAOM.
Results:
Serum total immunoglobulin levels were typically within the normal range, but there was a decrease in IgG2 in >20% of cases. Serotype-specific antibody titers were not significantly different for any serotype in the rAOM group compared with the healthy controls, except for serotype 3. Conversely, the ability of serotype-specific antibodies to induce opsonophagocytic killing was increased in the rAOM group for three serotypes (6 A, 6B, and 14). There was a positive correlation between the titer of antibodies and the opsonophagocytic killing activity in five serotypes (6 A, 6B, 9 V, 19 F, and 23 F).
Conclusion And Significance:
Quantitative and qualitative evaluation of serotype-specific antibodies would be an important method for evaluating humoral immunity in children with rAOM.
More Related Videos
09:20Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
Published on: April 28, 2014
11:32Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Published on: February 23, 2014
