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Advances in Online Comprehensive Two-Dimensional Liquid Chromatography Method Development.
Eugene H J Nell1, Magriet Muller1, André de Villiers1
1Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, South Africa;
Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|February 27, 2025
Summary
Comprehensive two-dimensional liquid chromatography (LC×LC) offers new insights into complex samples. Advanced method development strategies are improving the efficiency and accessibility of this powerful analytical technique.
Area of Science:
- Analytical Chemistry
- Chromatography Science
Background:
- Comprehensive two-dimensional liquid chromatography (LC×LC) is a powerful technique for analyzing complex samples.
- Widespread adoption is limited by complex method development and optimization challenges.
Purpose of the Study:
- To summarize advancements in online LC×LC method development.
- To highlight recent trends in optimizing LC×LC methods.
Main Methods:
- Review of progress in online LC×LC method development.
- Analysis of recent trends in advanced method optimization.
Main Results:
- Progress in online LC×LC method development has been summarized.
- Advanced optimization strategies are emerging.
Conclusions:
- Method development for LC×LC is complex due to numerous interdependent variables.
- Progress in optimization is enabling more efficient LC×LC methods.
Keywords:
LC×LCcomprehensive two-dimensional liquid chromatographymethod developmentonlineoptimizationMore Related Videos
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