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Published on: October 11, 2024
Quantifying Rhinovirus Genomic and Negative-Strand RNA by RT-PCR
Shahina Wiehler1, Michelle E Love1, David Proud2
1Department of Physiology and Pharmacology, and Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Abstract:
Molecular methods permit measurements of viral genomic RNA as well as of the negative-strand template generated during rhinovirus replication. As such, assays of negative-strand RNA can provide a means for relative quantification of levels of double-stranded RNA that is generated during replication and that interacts with pattern recognition receptors to activate the host immune response. Here we provide RT-PCR protocols for quantitative assays for rhinovirus genomic and negative-strand RNAs.

