Sensitive Assessment of Ketotifen Levels in Tablets and Plasma Using a Photoinduced Electron Transfer (PET)...

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Ketotifen (KTF) is an asthma medication used in conjunction with other treatments.
• Accurate quantification of KTF is crucial for therapeutic monitoring and quality control.

Purpose of the Study:

• To develop and validate a novel spectrofluorimetric method for KTF detection.
• To assess the method's applicability in pharmaceutical formulations and biological samples.

Main Methods:

• A photoinduced electron transfer (PET) inhibition spectrofluorimetric approach was employed.
• KTF was protonated using acetic acid, leading to enhanced fluorescence at 392 nm.
• The method was applied to KTF tablets and spiked human blood plasma samples.

Main Results:

• The method demonstrated sensitive KTF detection in the range of 50–500 ng mL⁻¹.
• Excellent detection and quantification limits were achieved (13.41 and 40.65 ng mL⁻¹, respectively).
• High percentage recoveries (98.40%–99.82%) in plasma samples confirmed precision and reliability; tablet content uniformity was also successfully evaluated.

Conclusions:

• The developed spectrofluorimetric method is sensitive, precise, and reliable for KTF quantification.
• This approach is suitable for analyzing KTF in both pharmaceutical products and biological matrices.
• The method offers a valuable tool for quality control and therapeutic drug monitoring of ketotifen.