A mitochondria targeted hydrogen peroxide fluorescent probe for monitoring oxidative stress during influenza virus infection
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Published on: June 2, 2023
Hao Chang1, Jianing Wang2, Yang Yang2
1Department of Laboratory Medicine, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, China; Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou 510632, China.
Abstract:
Hydrogen peroxide (H2O2), an important marker of oxidative stress, plays a significant role in infectious diseases. Oxidative stress induced by influenza virus infection is intricately linked to the pathological processes of host cells. In this work, a fluorescent probe QLC1 based on the coumarin was developed for fluorescence imaging of mitochondrial H2O2 level in host cells during influenza virus infection. The self-immolative reaction of QLC1 triggered by H2O2 will lead to a 250-fold fluorescence enhance and the limit of detection was determined to be 0.176 μM. Using this probe, we monitored oxidative stress during influenza infection and identified a link between redox status and influenza virus replication.
Insights
Researchers developed a fluorescent probe, QLC1, to measure hydrogen peroxide (H2O2) in cells during influenza virus infection. This tool revealed a connection between cellular redox status and viral replication, aiding in understanding infectious disease pathology.
Area of Science:
- Biochemistry
- Cell Biology
- Infectious Diseases
Background:
- Hydrogen peroxide (H2O2) is a key indicator of oxidative stress, significantly impacting host cell pathology during infectious diseases like influenza.
- Influenza virus infection induces oxidative stress, which is closely associated with the disease's pathological mechanisms.
Purpose of the Study:
- To develop a novel fluorescent probe for visualizing mitochondrial H2O2 levels in host cells during influenza virus infection.
- To investigate the relationship between cellular redox status and influenza virus replication using the developed probe.
Main Methods:
- Development of a coumarin-based fluorescent probe (QLC1) designed for H2O2 detection.
- Utilizing the probe for fluorescence imaging of mitochondrial H2O2 in host cells infected with influenza virus.
- Quantifying H2O2 levels and assessing the limit of detection (0.176 μM).
Main Results:
- The QLC1 probe exhibited a 250-fold fluorescence enhancement upon reaction with H2O2.
- Mitochondrial H2O2 levels were successfully monitored in host cells during influenza infection.
- A direct correlation was identified between the cellular redox state and the replication efficiency of the influenza virus.
Conclusions:
- The developed fluorescent probe QLC1 is effective for monitoring mitochondrial H2O2 levels and cellular oxidative stress in the context of influenza virus infection.
- Findings highlight the critical role of redox status in regulating influenza virus replication, offering potential therapeutic targets.
