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Protocol for converting assigned nuclear Overhauser enhancement spectroscopy series peaks into exact distance
Morkos A Henen1, David McCall2, David Jones1
1University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.
Abstract:
In the exact nuclear Overhauser enhancement (eNOE) method, tight upper and lower interatomic distance restraints for structure calculations and comparison are extracted from NMR nuclear Overhauser enhancement spectroscopy (NOESY) spectra of biomolecules recorded with different mixing times. Here, we present a protocol for exporting the resonance assignments of NOESY spectra from CCPNmr (v.2) into nmrDraw to obtain peak intensities. We then detail procedures for converting them into distances using either the CYANA- or MATLAB-based "exact NOE by relaxation matrix analysis" (eNORA) program.
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