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Updated: Jul 21, 2026

Analysis of DNA Double-strand Break DSB Repair in Mammalian Cells
Published on: September 8, 2010
Protocol for single-cell analysis of DNA double-strand break production and repair in cell-cycle phases by automated
Mathéa Geraud1, Lara Fernandez Martinez1, Andrea Carla Ajello1
1Cancer Research Center of Toulouse (CRCT), INSERM, Université de Toulouse, CNRS, 31037 Toulouse, France.
Abstract:
The mechanisms of DNA double-strand break (DSB) production and repair vary throughout the cell cycle. Here, we provide a protocol to quantify DSB production and repair in G1, S, and G2 phases of asynchronous adherent cells by coupling the staining of DSBs and cell-cycle markers with automated high-content fluorescence microscopy. We describe steps for cell seeding, treatment, staining, imaging, and analysis. This protocol is broadly applicable for monitoring DSB dynamics at single-cell level throughout the cell cycle. For complete details on the use and execution of this protocol, please refer to Geraud et al.1.
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