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Related Concept Videos

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Related Experiment Video

Updated: Jun 14, 2026

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores
09:32

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores

Published on: November 20, 2014

BOOST: a robust ten-fold expansion method on hour-scale.

Jinyu Guo1, Hui Yang1, Chixiang Lu1

  • 1Department of Chemistry, The University of Hong Kong, Pok Fu Lam, Hong Kong, China.

Nature Communications
|March 2, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces BOOST, a rapid expansion microscopy workflow. It significantly reduces sample processing time for enhanced visualization of biological specimens, including challenging FFPE sections.

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Universal Molecular Retention with 11-Fold Expansion Microscopy
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Related Experiment Videos

Last Updated: Jun 14, 2026

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores
09:32

Mechanical Expansion of Steel Tubing as a Solution to Leaky Wellbores

Published on: November 20, 2014

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics
14:14

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics

Published on: April 16, 2017

Universal Molecular Retention with 11-Fold Expansion Microscopy
10:31

Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

Area of Science:

  • Biotechnology
  • Microscopy
  • Cell Biology

Background:

  • Expansion microscopy (ExM) improves imaging resolution by physically enlarging biological samples.
  • Current ExM methods face limitations in processing time, especially for difficult samples like FFPE tissues and large 3D specimens.
  • There is a need for faster and more robust ExM protocols.

Purpose of the Study:

  • To develop a rapid and robust expansion microscopy workflow named BOOST.
  • To overcome the time-consuming nature of existing ExM protocols.
  • To enable high-fold expansion of challenging biological specimens.

Main Methods:

  • BOOST utilizes microwave-accelerated chemistry for rapid sample expansion.
  • The workflow involves a single-step expansion process.
  • BOOST is designed to be adaptable using common and stable reagents.

Main Results:

  • BOOST achieves a 10-fold expansion of cultured cells, tissue sections, and FFPE sections in under 90 minutes.
  • This method successfully expands large, millimeter-sized 3D specimens, a previously unattainable feat.
  • The workflow demonstrates robustness and speed compared to existing ExM techniques.

Conclusions:

  • BOOST offers a significantly accelerated and effective expansion microscopy solution.
  • The technique broadens the applicability of ExM, particularly for challenging sample types.
  • BOOST has the potential to increase the adoption and utility of expansion microscopy in research.