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Updated: May 24, 2025

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
Published on: November 1, 2024
PDRG1 is essential for early plant development as a component of the prefoldin-like complex
Laura Hernández-Villa1, Alberto Palacios-Abella1, Yaiza Gómez-Mínguez1
1Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Valencia, Spain.
None:
p53 AND DNA DAMAGE-REGULATED GENE1 (PDRG1) is part of the prefoldin-like complex (PFDLc) in plants and animals. Whether PDRG1 acts primarily as a subunit of PFDLc or as an independent subunit is not known in any eukaryote. Here, we show that impairment of PDRG1 activity in Arabidopsis thaliana leads to embryonic lethality, as is the case for the other prefoldin-like proteins UXT and AtURI. The subunits of PFDLc are the main interactors of PDRG1 in vivo, and the interactomes of PDRG1, UXT, and AtURI show strong overlaps, including subunits of nuclear RNA polymerases and various complexes of the spliceosome. Our results show that PDRG1 plays an essential role in Arabidopsis mainly as a subunit of PFDLc.
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