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Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
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Piperine as Potential Anti-Inflammatory Agent: In-Silico Studies Along With In-Vitro Anti-Cathepsin B Activity, and

Manishita R Sharma1, Parveen K Sharma2, Brij Mohan3

  • 1Department of Chemistry, Kurukshetra University, Kurukshetra, India.

Chemistry & Biodiversity
|March 3, 2025
PubMed
Summary

Piperine shows significant anti-inflammatory effects, matching commercial drugs in silico. In vitro studies confirm its potential by inhibiting cathepsin B and protecting proteins.

Keywords:
anti‐cathepsin b activity | anti‐inflammatory targets | biological activities | molecular docking | piperine

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Area of Science:

  • Pharmacology
  • Computational Chemistry
  • Biochemistry

Background:

  • Piperine, a natural compound, possesses various pharmacological properties.
  • Its anti-inflammatory potential has been investigated against several molecular targets.

Purpose of the Study:

  • To screen piperine against anti-inflammatory targets using computational methods.
  • To evaluate in vitro anti-cathepsin B and serum protein-protecting activities of piperine.

Main Methods:

  • In silico screening using computational tools (vNN-ADMET, SWISS ADME, Spartan-14, iGemdock, DS-Visualizer).
  • Evaluation of anti-cathepsin B activity.
  • Assessment of serum protein anti-denaturation activity.

Main Results:

  • Piperine exhibited comparable anti-inflammatory potential to ibuprofen and naproxen in silico.
  • Complete inhibition of cathepsin B was observed at 200 µM piperine.
  • 100% anti-denaturation of serum proteins was observed at 10 µM piperine.

Conclusions:

  • Piperine demonstrates significant anti-inflammatory potential validated by both in silico and in vitro studies.
  • Piperine targets previously unreported mechanisms for its anti-inflammatory action.