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Updated: May 24, 2025

Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
A Systems Biology Approach of Quantifying Signal Transduction to B-Cell Proliferation and Differentiation
Koushik Roy1, Aimilia Vareli2, Simon Mitchell3
1Division of Microbiology and Immunology, Department of Pathology, University of Utah, Salt Lake City, UT, USA. koushik.roy@path.utah.edu.
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Combining mathematical modeling with experiments enables quantitative understanding of cell signaling, transcriptional regulation, and cell fate decisions. Here, we provide a systems biology approach to link signal transduction with B cells fate decisions, to enable quantitative prediction of B-cell proliferation, and differentiation. We describe methodology to run simulations that reveal how signal transduction regulates gene expression and predicts cell fate decision. We describe how to quantitively validate modeling predictions with wet-lab experiments.
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