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Updated: May 24, 2025
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
Published on: July 3, 2014
Synergistic microglial modulation by laminarin-based platinum nanozymes for potential intracerebral hemorrhage
Xiumei Guo1, Qionghua Zheng2, Wen Gao3
1Department of Neurosurgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China; Laboratory of Clinical Pharmacy, Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China; Medical Center for Neurological Disorders, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province, 362000, China; Department of Neurology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, China.
Abstract:
Abnormal microglial activation increases inflammation, causing significant brain damage after intracerebral hemorrhage (ICH). To aid recovery, treatments should regulate oxidative stress and inhibit the M1-like phenotype (pro-inflammation) of microglia. Recently, antioxidant nanozymes have emerged as tools for modulating microglial states, but detailed studies on their role in ICH treatment are limited. To address this, we developed an ultra-small (3-4 nm) laminarin-modified platinum nanozyme (Pt@LA) for the synergistic regulation of microglial polarization, offering a novel therapeutic strategy for ICH. Pt@LA effectively scavenges reactive oxygen species (ROS) through superoxide dismutase (SOD) and catalase (CAT)-like activities. Laminarin may inhibit the Dectin-1 receptor on microglia and its inflammatory pathway, Syk/NF-κB, reducing neuroinflammation. In vitro, Pt@LA decreased pro-inflammatory microglia and cytokine expression by inhibiting the Dectin-1/Syk/NF-κB and ROS-mediated NF-κB pathways. Furthermore, Pt@LA protected neurons, inhibited glial scar formation, and improved neurological function in ICH rats. Overall, this study presents Pt nanozymes based on naturally extracted laminarin and explores their application in alleviating oxidative stress and neuroinflammation after ICH, bridging nanozyme research and neuroscience.
Insights
This study introduces a novel platinum nanozyme (Pt@LA) that reduces brain inflammation and oxidative stress after intracerebral hemorrhage (ICH). Pt@LA effectively regulates microglial polarization, offering a promising new therapeutic strategy for ICH recovery.
Area of Science:
- Neuroscience
- Biomaterials Science
- Nanotechnology
Background:
- Abnormal microglial activation drives inflammation and brain damage in intracerebral hemorrhage (ICH).
- Effective ICH treatments require regulating oxidative stress and inhibiting pro-inflammatory M1-like microglia.
- Antioxidant nanozymes show potential for modulating microglial states, but their role in ICH is underexplored.
Purpose of the Study:
- To develop and evaluate an ultra-small laminarin-modified platinum nanozyme (Pt@LA) for synergistic regulation of microglial polarization in ICH.
- To investigate Pt@LA's capacity to alleviate oxidative stress and neuroinflammation post-ICH.
- To establish a novel therapeutic strategy for ICH leveraging nanozyme technology.
Main Methods:
- Development of ultra-small (3-4 nm) laminarin-modified platinum nanozymes (Pt@LA).
- In vitro assessment of Pt@LA's antioxidant (SOD and CAT-like) and anti-inflammatory effects on microglia, including inhibition of Dectin-1/Syk/NF-κB and ROS-mediated NF-κB pathways.
- In vivo evaluation of Pt@LA in ICH rat models, assessing neuronal protection, glial scar formation, and neurological function.
Main Results:
- Pt@LA demonstrated effective scavenging of reactive oxygen species (ROS) via superoxide dismutase (SOD) and catalase (CAT)-like activities.
- In vitro studies showed Pt@LA reduced pro-inflammatory microglia and cytokine expression by inhibiting key inflammatory pathways.
- In ICH rats, Pt@LA treatment protected neurons, reduced glial scar formation, and improved neurological outcomes.
Conclusions:
- Pt@LA acts as a potent antioxidant and anti-inflammatory agent by modulating microglial polarization through Dectin-1/Syk/NF-κB and ROS-mediated NF-κB pathways.
- The developed nanozyme offers a promising therapeutic approach for mitigating oxidative stress and neuroinflammation in ICH.
- This study bridges nanozyme research and neuroscience, presenting a novel strategy for ICH treatment.
