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Updated: May 24, 2025
A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes
Published on: February 17, 2023
Protective Effect of Medicago sativa L. (Fabaceae) against Excision and Incision Wound Models in Diabetic Rats
Phool Chandra1, Mohammad Kaleem2, Neetu Sachan3
1Department of Pharmacology, Teerthanker Mahaveer College of Pharmacy, Teerthanker Mahaveer University, Moradabad, U.P, 244001, India.
Introduction:
Medicago sativa (M. sativa), commonly known as Alfalfa, is a herb from the Fabaceae family that has a long history of being used to treat digestive, diabetic, and blood disorders, as well as to support liver health. The objective was to evaluate the effects of ethanolic extract of M. sativa (EEMS) on wounds in normal rats or alloxan hydrate-induced diabetic rats.
Methods:
The wounds were created by excision (n=30) and incision (n=30) in rats. The Group IIV were diabetic rats treated with simple ointment BP, 10% weight-based povidone-iodine (10% PI), ointment of 5% w/w EEMS (5% w/w OEEMS), and 10% w/w EEMS (10% w/w OEEMS). Group 1 acted as a control and was treated with simple ointment BP. The wound area in the diabetic control groups was 292.33 ± 0.8 mm2 on the 18th day.
Results:
Rats treated with 10% PI, 5% OEEMS, and 10% OEEMS showed a significant reduction in wound area of 68.33 ± 1.29, 248.33 ± 1.30, and 61 ± 1.91 mm2, respectively, on the 18th day as compared to the control group. Rats treated with 10% PI, 5% w/w OEEMS, and 10% w/w OEEMS showed a significant increment in wound-breaking strength, respectively, as compared to diabetic rats on day 10 in the incision wound model.
Conclusion:
The results demonstrated that the OEEMS has potent wound-healing properties in diabetic rats.
