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Updated: May 24, 2025
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Published on: July 17, 2019
Interference with Immunoassays of a Neonate on High Biotin: Case Report and Literature Review
Naeema Al Shibli1, Azza Al Shidhani1, Irfan Ullah1
1Department of Child Health, Sultan Qaboos University Hospital, Muscat, Oman.
Abstract:
Biotin is sometimes administered in mega doses to children to treat certain inborn errors of metabolism. We report the case of a one-week-old newborn who was started on a 'mitochondrial cocktail' that contained a high dose of biotin. On day seven of life, his thyroid function test showed a biochemical picture of primary hyperthyroidism, which was not clinically evident. The suspicion of immunoassay interference with thyroid function test was confirmed when another lab, using a different immunoassay, gave normal results. If the biochemical profile does not match the clinical picture, it is reasonable to doubt the test result and think of assay interference.
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