Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reliability and Validity01:29

Reliability and Validity

12.7K
Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
12.7K
Measures of Intelligence01:29

Measures of Intelligence

6.0K
Psychologists measure intelligence by using standardized tests that produce a score known as the intelligence quotient or IQ. To understand IQ tests, it's important to recognize the key principles behind their construction: validity, reliability, and standardization.
Validity refers to how well a test measures what it claims to measure. An intelligence test should accurately assess intelligence rather than another characteristic, like anxiety. Criterion validity is one way to evaluate this;...
6.0K
Self-Report Tests of Personality01:22

Self-Report Tests of Personality

293
Self-report inventories are objective personality assessments that use multiple-choice items or numbered scales, typically ranging from 1 (strongly disagree) to 5 (strongly agree). They are often called Likert scales after Rensis Likert. These inventories are widely used due to their ease of administration and cost-effectiveness. One of the most prominent examples is the Minnesota Multiphasic Personality Inventory (MMPI), initially developed in the 1940s to assess abnormal personality traits.
293
Testing a Claim about Standard Deviation01:19

Testing a Claim about Standard Deviation

2.4K
A complete procedure to test a claim about population standard deviation or population variance is explained here.
The hypothesis testing for the claim of population standard deviation (or variance) requires the data and samples to be random and unbiased. The population distribution also must be normal. There is no specific requirement on the sample size as the estimation is based on the chi-square distribution.
As a first step, the hypothesis (null and alternative) concerning the claim about...
2.4K
Humanistic Psychology01:24

Humanistic Psychology

1.0K
Humanistic psychology emerged in the mid-20th century as a response to the deterministic and pessimistic nature of behaviorism and psychoanalysis. While behaviorism focused on observable behaviors influenced by the environment and psychoanalysis delved into unconscious motivations, both theories suggested that human actions lacked free will. In contrast, humanistic psychology offers a perspective that emphasizes the innate potential for goodness and growth within every individual.
This approach...
1.0K
Friedman Two-way Analysis of Variance by Ranks01:21

Friedman Two-way Analysis of Variance by Ranks

130
Friedman's Two-Way Analysis of Variance by Ranks is a nonparametric test designed to identify differences across multiple test attempts when traditional assumptions of normality and equal variances do not apply. Unlike conventional ANOVA, which requires normally distributed data with equal variances, Friedman's test is ideal for ordinal or non-normally distributed data, making it particularly useful for analyzing dependent samples, such as matched subjects over time or repeated measures...
130

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Risk factors on the unintentional injuries among rural children aged 0-12 in Shaanxi province].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2013
Same author

Adcyap1r1 genotype, posttraumatic stress disorder, and depression among women exposed to childhood maltreatment.

Depression and anxiety·2013
Same author

Current status and challenge of Human Parasitology teaching in China.

Pathogens and global health·2012
Same author

Molecular characterization of heterogeneous mesenchymal stem cells with single-cell transcriptomes.

Biotechnology advances·2012
Same author

Surgical treatment of ossification of the ligamentum flavum associated with dural ossification in the thoracic spine.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2012
Same author

Broadband focusing ultrasonic transducers based on dimpled LiNbO3 plate with inversion layer.

IEEE transactions on ultrasonics, ferroelectrics, and frequency control·2012

Related Experiment Video

Updated: May 24, 2025

Assessment of Child Anthropometry in a Large Epidemiologic Study
09:36

Assessment of Child Anthropometry in a Large Epidemiologic Study

Published on: February 2, 2017

27.0K

Evaluative Methodology for HRD Testing: Development of Standard Tools for Consistency Assessment.

Zheng Jia1, Yaqing Liu2, Shoufang Qu1

  • 1Department of In Vitro Diagnostic Reagent, National Institutes for Food and Drug Control (NIFDC), Beijing 100050, China.

Genomics, Proteomics & Bioinformatics
|March 4, 2025
PubMed
Summary

Standardized reference materials and datasets were developed to evaluate homologous recombination deficiency (HRD) testing. This initiative addresses inconsistencies in current assays, aiming to improve the reliability of HRD biomarkers in oncology.

Keywords:
Homologous recombination deficiencyPerformance evaluationPrecision medicineReference datasetReference material

More Related Videos

Development of a Virtual Reality Assessment of Everyday Living Skills
10:32

Development of a Virtual Reality Assessment of Everyday Living Skills

Published on: April 23, 2014

18.3K
Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties
12:55

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties

Published on: September 27, 2020

8.3K

Related Experiment Videos

Last Updated: May 24, 2025

Assessment of Child Anthropometry in a Large Epidemiologic Study
09:36

Assessment of Child Anthropometry in a Large Epidemiologic Study

Published on: February 2, 2017

27.0K
Development of a Virtual Reality Assessment of Everyday Living Skills
10:32

Development of a Virtual Reality Assessment of Everyday Living Skills

Published on: April 23, 2014

18.3K
Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties
12:55

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties

Published on: September 27, 2020

8.3K

Area of Science:

  • Oncology
  • Genomics
  • Biomarker Development

Background:

  • Homologous recombination deficiency (HRD) is a key prognostic and predictive biomarker in cancer.
  • Current HRD testing methods, particularly targeted panels, yield inconsistent results, necessitating standardized benchmarks.
  • There is an urgent need for reliable evaluation tools for HRD assay performance.

Purpose of the Study:

  • To develop and validate standardized reference materials and datasets for homologous recombination deficiency (HRD) testing.
  • To establish benchmarks for evaluating the accuracy and reproducibility of HRD assays.
  • To identify challenges in HRD detection and improve assay consistency.

Main Methods:

  • Development of ten pairs of DNA reference materials from cancer and normal cell lines with varying cancer-to-normal mass ratios.
  • Creation of reference datasets for allele-specific copy number variations (ASCNVs) and HRD scores using multiple sequencing methods and analytical pipelines.
  • Validation of four HRD panel assays using the established reference materials and datasets.

Main Results:

  • Reference datasets for ASCNVs and HRD scores were established, covering a significant portion of the human genome.
  • Genomic Instability Scores (GIS) ranged from 11 to 96, suitable for various validation thresholds.
  • Significant variability was observed in HRD panel assays regarding ASCNV detection and HRD scores, with limited concordance to reference data.

Conclusions:

  • The developed HRD reference materials and datasets provide a robust toolkit for objective HRD assay evaluation.
  • The study highlights significant variability in current HRD testing, underscoring the need for standardization.
  • Addressing challenges in ASCNV detection and the impact of high ploidy is crucial for improving HRD assay consistency.