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Related Experiment Videos

Donor eye contamination.

R J Olson, M E McMain, T E Slappey

    Annals of Ophthalmology
    |December 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Donor eye cultures revealed bacterial contamination, particularly in anterior chamber samples. This highlights the need for rigorous eye bank culturing protocols to prevent corneal infections in transplant recipients.

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    Cornea·2001

    Area of Science:

    • Ophthalmology
    • Microbiology
    • Transplantation Science

    Background:

    • Donor eye contamination poses a risk for post-keratoplasty infections.
    • Chronic debilitating diseases may be associated with terminal bacteremia, increasing donor contamination risk.

    Purpose of the Study:

    • To assess the microbial contamination rates in donor eyes using different culture methods.
    • To evaluate the effectiveness of current culturing protocols in preventing infections.

    Main Methods:

    • Collected 127 cultures from donor eyes in McCarey-Kaufman (M-K) media.
    • Performed 60 anterior chamber cultures from whole donor eyes.
    • Monitored 16 eyes undergoing corneal transplantation for infection.

    Main Results:

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    • 17 out of 127 donor eyes showed positive cultures.
    • 13 positive cultures originated from anterior chamber samples, while 4 were from M-K media.
    • One successfully treated clinical corneal infection occurred in the 16 transplanted eyes.

    Conclusions:

    • Anterior chamber cultures are more sensitive in detecting donor eye contamination.
    • Implementing routine anterior chamber cultures for all donor eyes is recommended.
    • Prompt communication of positive culture results to surgeons is crucial for patient safety.