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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Enzyme-linked Receptors01:00

Enzyme-linked Receptors

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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Transducer Mechanism: Enzyme-Linked Receptors01:27

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Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
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Covalently Linked Protein Regulators02:04

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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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Enzymes02:34

Enzymes

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Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
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Fibronectins Connect Cells with ECM01:25

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Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
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Updated: Jan 30, 2026

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
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Enzyme-linked immunosorbent assay to quantify fibronectin.

M J Gomez-Lechon, J V Castell

    Analytical Biochemistry
    |February 15, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a new assay to measure fibronectin, a key protein in body fluids. This method utilizes fibronectin

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    Area of Science:

    • Biochemistry
    • Protein Analysis
    • Assay Development

    Background:

    • Fibronectin is a multifunctional soluble glycoprotein present in blood and other bodily fluids.
    • Fibronectin exhibits binding affinity to various biological substrates like collagen and gelatin.
    • Notably, fibronectin strongly adsorbs to polystyrene plastic surfaces, resisting denaturation.

    Purpose of the Study:

    • To develop a novel, noncompetitive enzyme-linked immunosorbent assay (ELISA) for quantifying soluble fibronectin.
    • To leverage the unique binding property of fibronectin to polystyrene for assay development.

    Main Methods:

    • A three-step noncompetitive ELISA was designed based on fibronectin's selective adsorption to polystyrene.
    • Key assay parameters, including selective attachment, protein interference, linearity, and sensitivity, were evaluated.
    • The assay was applied to measure fibronectin concentrations in biological samples from various animal sources.

    Main Results:

    • Fibronectin demonstrated rapid and strong binding to polystyrene, which was stable against denaturing agents.
    • The developed ELISA showed a linear response and a defined limit of sensitivity for fibronectin quantification.
    • The assay successfully measured fibronectin levels in diverse biological samples.

    Conclusions:

    • A robust and sensitive ELISA method for quantifying soluble fibronectin has been established.
    • The assay's foundation on fibronectin's stable adsorption to polystyrene offers a reliable detection strategy.
    • This method is applicable for measuring fibronectin across different animal species.