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Updated: May 24, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Cryo-STEM tomography for cell biology using thick lamella
Kazuhiro Aoyama1,2, Hiroko Takazaki3, Misaki Arie3
1NanoPort Japan Application Laboratory, Thermo Fisher Scientific, Shinagawa Seaside West Tower 1F, 4-12-2 Higashi-Sinagawa, Shinagawa-ku, Tokyo 140-0002, Japan.
Abstract:
Electron tomography is a powerful tool for structural studies in cell biology, but specimen thickness remains a significant limitation. Scanning transmission electron microscopy (STEM) tomography offers advantages in this regard. Recent developments in focused ion beam slicing methods for cryo-cell biology have enabled the observation and 3D reconstruction of relatively thick specimens (300-500 nm) using cryo-STEM tomography. Organelles such as mitochondria and the nuclear membrane have been clearly reconstructed, demonstrating the promise of STEM tomography for structural studies in cell biology.
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