Unipolar and bipolar signal acquisition to distinguish artifacts from nervous activity

The acquisition of nervous activity is a meticulous work which requires particular attention to validate the presence of the signal of interest and to distinguish it from electrical and physiological artifacts which are constantly added to the recordings even when the experimenters have taken care to clean the measurement environment as much as possible. In this article, we propose to analyze the bipolar and unipolar recordings from the same electrode contacts, to confirm the nervous nature of the recorded signal and distinguish it from artifacts on the vagus nerve. The metrics used for the analysis of these signals confirmed the recording of nervous activity and the ability of the measuring device to distinguish and separate the respiratory muscular artifact from the respiratory nervous activity although they have very similar shapes.