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Improving Bacillus subtilis as Biological Chassis Performance by the CRISPR Genetic Toolkit
Xianhai Cao1,2, Xiaojuan Wang2,3, Ruirui Chen2,3
1Instrumental Analysis and Research Center, Tianjin University of Traditional Chinese Medicine, 301617 Tianjin, China.
ACS Synthetic Biology
|March 5, 2025
Summary
CRISPR technology has been significantly upgraded for Bacillus subtilis, enhancing its use in gene editing and biological production. This advancement aims to make B. subtilis a leading industrial microorganism.
Area of Science:
- Microbiology
- Biotechnology
- Synthetic Biology
Background:
- Bacillus subtilis is a key Gram-positive bacterium for industrial applications.
- Its synthetic biology and metabolic engineering lag behind E. coli and S. cerevisiae.
- CRISPR technology offers efficient, programmable genome editing.
Purpose of the Study:
- Review recent CRISPR toolkit advancements in B. subtilis.
- Discuss CRISPR-based engineering for biochemical and protein production.
- Provide perspectives on CRISPR applications in B. subtilis.
Main Methods:
- Review of recent literature on CRISPR technology in B. subtilis.
- Analysis of CRISPR applications in gene editing, transcriptional regulation, and enzyme modulation.
- Discussion of advances in B. subtilis chassis engineering.
Main Results:
- CRISPR technology has been intensely upgraded in B. subtilis since 2016.
- New CRISPR tools enable gene editing, transcriptional regulation, and enzyme modulation.
- CRISPR engineering advances efficient biochemical and protein production in B. subtilis.
Conclusions:
- CRISPR-based biotechnology presents significant opportunities for B. subtilis.
- Further development can position B. subtilis as a leading industrial microorganism.
- Addressing current challenges will enhance its competitiveness with E. coli and S. cerevisiae.
Keywords:
Bacillus subtilisCRISPR/Casbase editorgenome editingmetabolic regulationmicrobial productionMore Related Videos
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