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Genome Sequence of Mycobacterium Phage Guppsters

Emma K Alley1, Lexi C Hill1, Mya E Houglum1

  • 1College of St. Scholastica, Duluth, Minnesota, United States.

Micropublication Biology
|March 5, 2025
PubMed

Insights

Mycobacterium Phage Guppsters, a siphovirus, was isolated from Mycobacterium smegmatis. Unlike other F1 cluster phages, Guppsters lacks mobile genetic elements in its genome.

Area of Science:

  • Bacteriology
  • Virology
  • Genomics

Background:

  • Mycobacteriophages are viruses that infect Mycobacterium bacteria.
  • The F1 cluster is a group of mycobacteriophages with shared genetic characteristics.
  • Mobile genetic elements can influence phage evolution and host interactions.

Purpose of the Study:

  • To characterize the novel mycobacteriophage Guppsters.
  • To determine the genomic features of Guppsters, particularly the presence of mobile elements.
  • To understand the phylogenetic placement of Guppsters within the F1 cluster.

Main Methods:

  • Isolation and characterization of mycobacteriophage Guppsters from Mycobacterium smegmatis.
  • Genome sequencing and analysis to determine genome size and gene content.
  • Comparative genomics to assess similarity with other phages and identify mobile elements.

Main Results:

  • Mycobacterium Phage Guppsters was successfully isolated and identified as a siphovirus.
  • Guppsters possesses a 54,835 base pair genome.
  • Phylogenetic analysis placed Guppsters in cluster F1, but it uniquely lacks mobile genetic elements, unlike most F1 phages.

Conclusions:

  • Mycobacteriophage Guppsters represents a distinct lineage within the F1 cluster.
  • The absence of mobile elements in Guppsters offers insights into phage genome evolution.
  • Further research on Guppsters may elucidate the role of mobile elements in mycobacteriophage-host interactions.

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