Related Experiment Video
Updated: May 24, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
DNA aptamers targeting P. aeruginosa RNAP
Hari Timilsina1, Dmytro Kompaniiets2, Satya Prakash Arya1
1Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH, USA. tanx@bgsu.edu.
Abstract:
We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandem purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.
More Related Videos
07:59Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
11:35Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018