Related Experiment Video

Updated: May 24, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.6K

Complete genome sequence of Escherichia coli phage Eryne

Valentin Druelle1, Alexander Harms1,2

  • 1Biozentrum, University of Basel, Basel, Switzerland.

Microbiology Resource Announcements
|March 5, 2025
PubMed

Insights

A new bacteriophage, Eryne, infects Escherichia coli by targeting surface sugars. Its genome was sequenced, revealing it belongs to the Justusliebigvirus genus, known for broad host recognition.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria.
  • Escherichia coli is a common bacterium with clinical and laboratory relevance.
  • Phage host recognition is often mediated by interactions with bacterial surface components.

Purpose of the Study:

  • To characterize the novel bacteriophage Eryne.
  • To determine the genome sequence of phage Eryne.
  • To classify phage Eryne within existing viral taxonomy.

Main Methods:

  • Genome sequencing of bacteriophage Eryne.
  • Bioinformatic analysis of the phage genome.
  • Phylogenetic analysis to determine genus and subfamily classification.

Main Results:

  • The complete genome sequence of bacteriophage Eryne (145,026 bp) was determined.
  • Phage Eryne infects both clinical and laboratory strains of Escherichia coli.
  • Eryne was classified into the genus Justusliebigvirus within the subfamily Stephanstirmvirinae.
  • The genus Justusliebigvirus is characterized by multivalent host recognition.

Conclusions:

  • Bacteriophage Eryne represents a newly identified virus with potential applications in targeting Escherichia coli.
  • The genomic data places Eryne within a group of phages known for their ability to recognize multiple hosts.
  • Further research can explore Eryne's specific mechanisms of glycan targeting and host range.
Keywords:
Escherichia coliJustusliebigvirusbacteriophagetailspike

More Related Videos

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

4.9K
Novel Sequence Discovery by Subtractive Genomics09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.6K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.6K
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
752.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
86.9K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
10.9K
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K