[Experimental studies with the use of metastatic clones isolated from mouse tumors]

Low and high metastatic clones were isolated from mouse colon 26 by repeated cloning of tumor cells in the lung. These clone cells showed an interesting metastatic capacities, low and high lung metastasis. The results indicate that original tumor consisted of various and heterogeneous cell clones having different metastatic capacities. Using highly metastatic clones, NL-17 and B16-F10 and B16-BL6, adriamycin and 5-fluorouracil were found similarly effective to both parental and cloned tumor lines. Although there are several reports to indicate heterogeneous drug sensitivity of metastatic clones from the parental line, present study indicated the similar drug sensitivity. Therefore, heterogeneity of drug sensitivity is scheduled to check with different antitumor drugs.