Recalibrating Olfactory Neuroscience to the Range of Naturally Occurring Odor Concentrations

Sensory systems enable organisms to detect and respond to environmental signals relevant for their survival and reproduction. A crucial aspect of any sensory signal is its intensity; understanding how sensory signals guide behavior requires probing sensory system function across the range of stimulus intensities naturally experienced by an organism. In olfaction, defining the range of natural odorant concentrations is difficult. Odors are complex mixtures of airborne chemicals emitting from a source in an irregular pattern that varies across time and space, necessitating specialized methods to obtain an accurate measurement of concentration. Perhaps as a result, experimentalists often choose stimulus concentrations based on empirical considerations rather than with respect to ecological or behavioral context. Here, we attempt to determine naturally relevant concentration ranges for olfactory stimuli by reviewing and integrating data from diverse disciplines. We compare odorant concentrations used in experimental studies in rodents and insects with those reported in different settings including ambient natural environments, the headspace of natural sources, and within the sources themselves. We also compare these values to psychophysical measurements of odorant detection threshold in rodents, where thresholds have been extensively measured. Odorant concentrations in natural regimes rarely exceed a few parts per billion, while most experimental studies investigating olfactory coding and behavior exceed these concentrations by several orders of magnitude. We discuss the implications of this mismatch and the importance of testing odorants in their natural concentration range for understanding neural mechanisms underlying olfactory sensation and odor-guided behaviors.