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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

Updated: May 24, 2025

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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3D printed miniature attenuated total internal reflection assisted fluorescence microscopy.

Dong Hee Park1, Bin Chan Joo1, Kyu Ri Choi1

  • 1Department of Physics, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea.

Scientific Reports
|March 5, 2025
PubMed
Summary
This summary is machine-generated.

A new 3D-printed miniature attenuated total internal reflectance fluorescence (mini-ATIRF) microscope offers brighter images and is 73% lighter and 95% cheaper than conventional systems.

Keywords:
3D printingAttenuated total internal reflectionFluorescence microscopy

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Area of Science:

  • Scientific imaging
  • Biotechnology
  • Analytical chemistry

Background:

  • Advanced imaging and sensing are vital for analyzing cells, DNA, RNA, food, drugs, and forensics.
  • Conventional microscopy instruments are often complex, heavy, and not field-portable.
  • Emerging portable and field-ready instruments offer alternatives to traditional lab equipment.

Purpose of the Study:

  • To demonstrate a novel miniature attenuated total internal reflectance fluorescence (mini-ATIRF) microscope.
  • To leverage 3D printing for cost-effective and lightweight instrument fabrication.
  • To evaluate the performance of the mini-ATIRF microscope compared to conventional fluorescence microscopy.

Main Methods:

  • Utilized 3D printing technology for the fabrication of the miniature microscope.
  • Employed evanescent field illumination for excitation.
  • Incorporated surface plasmon-coupled emission for enhanced signal detection.

Main Results:

  • The developed mini-ATIRF microscope is 73% lighter than conventional fluorescence microscopes.
  • Achieved fluorescence images that are four times brighter than those from traditional systems.
  • Manufacturing costs were reduced by over 95% compared to conventional equipment.

Conclusions:

  • The 3D-printed mini-ATIRF microscope provides a portable, cost-effective, and high-performance alternative for fluorescence imaging.
  • This technology has significant potential for field-ready applications in various scientific disciplines.
  • The study highlights the advantages of additive manufacturing in developing advanced scientific instrumentation.