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Exploring cadmium uptake pathways in the roots of Coptis chinensis Franch
Yuting Niu1, Shuang He2, Lu Xing2
1College of Medicine, Shaanxi Institute of International Trade and Commerce, Xi'an, 710075, China.
Abstract:
To investigate the cadmium (Cd) uptake mechanism in the roots of Coptis chinensis Franch., we utilized non-invasive micro-test technology (NMT) to analyze Cd2+ and H+ fluxes, alongside ICP-MS to assess Cd content and fluxes under various inhibitors and ion channel blockers. Results revealed that Cd2+ uptake primarily occurs in the root meristematic zone. Cd2+ addition significantly inhibited H+ influx. The P-type ATPase inhibitor Na3VO4 and the metabolism inhibitor CCCP both reduced Cd2+ fluxes and Cd content in roots of C. chinensis Ca2+ channel blocker LaCl3 exhibited more pronounced inhibition on Cd2+ flux than K+ channel blocker TEA, indicating Cd2+ uptake mainly via Ca2+ channels. Therefore, active transport and Ca2+ channels may play an important role in Cd uptake in C. chinensis roots.
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