Related Experiment Video
Updated: May 24, 2025
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Published on: August 11, 2017
15.7K
Biomimetic integration of functionally controlled modular tissue building blocks for engineering 3D vascularized
Sangmin Lee1,2,3, Jeongbok Lee1,4, Hyunseok Kwon1,4
1Department of Bioengineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Republic of Korea.
Bioactive Materials
|March 6, 2025
Summary
This study engineered vascularized adipose tissue using novel nanofibers and spheroids. The method successfully coupled fat cell development and blood vessel formation for potential tissue reconstruction therapies.
Area of Science:
- Tissue Engineering
- Biomaterials Science
- Regenerative Medicine
Background:
- Adipose tissue vascularization is vital for homeostasis and energy storage.
- Existing 3D vascularized adipose tissue engineering methods face challenges with suppressed endothelial function during adipogenesis.
- A novel approach is needed to couple adipogenesis and vasculogenesis effectively for engineered adipose tissue.
Purpose of the Study:
- To develop a novel 3D in vitro system for reconstructing vascularized adipose tissue.
- To couple adipogenesis and vasculogenesis concurrently for enhanced tissue function.
- To create an in vitro model of obesity-related adipose tissue dysfunction.
Main Methods:
- Developed adipo-inductive nanofibers (ID/F@INS) containing indomethacin and insulin.
- Created adipogenic spheroids (AS) using human adipose-derived stem cells (hADSCs) and ID/F@INS within GelMA hydrogels.
- Generated vascular spheroids (VS) using hADSCs and human umbilical vein endothelial cells, then integrated AS and VS in GelMA hydrogels.
Main Results:
- ID/F@INS significantly enhanced in vitro adipogenesis of hADSCs in AS.
- Larger adipogenic spheroids showed greater adipogenesis.
- Integration of AS and VS (2:1 ratio) significantly improved vascular network formation, indicating concurrent adipogenesis and vasculogenesis stimulation.
- The system modeled obesity-like white adipose dysfunction in vitro.
- Engineered tissue showed vascularization and adipogenesis in vivo after implantation in mice.
Conclusions:
- The developed in vitro system effectively couples adipogenesis and vasculogenesis using inductive nanofibers and spheroid integration.
- This platform can model adipose tissue dysfunction and demonstrates potential for therapeutic applications in tissue reconstruction.
