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Related Experiment Video

Updated: Jun 14, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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Ultra-Specific G-Quadruplex-Colistin Interaction for Efficient Transcriptome-Wide G4 Mapping.

Shijiong Wei1, Xiaobo Zhang1, Yilong Feng2

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Journal of the American Chemical Society
|March 6, 2025
PubMed
Summary
This summary is machine-generated.

The antibiotic colistin (COL) specifically binds parallel G-quadruplexes (G4s), causing them to aggregate. This discovery enabled the development of CoRP-seq, a simple method to map RNA G4s in human cells.

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Area of Science:

  • Chemical Biology
  • Genomics
  • Molecular Biology

Background:

  • G-quadruplexes (G4s) are complex nucleic acid structures with dynamic polymorphism, posing challenges for chemical biology interventions.
  • Targeting G4s is crucial for understanding cellular processes and developing novel therapeutics.

Purpose of the Study:

  • To investigate the interaction of small molecules with specific G4 structures.
  • To develop a novel method for assessing the prevalence of RNA G-quadruplexes in the human transcriptome.

Main Methods:

  • Exploited the specific interaction between the antibiotic colistin (COL) and parallel G-quadruplexes (G4s).
  • Developed the COL-induced RNA G4 precipitation and sequencing (CoRP-seq) protocol, utilizing structure-specific aggregation and centrifugation for G4 isolation.
  • Applied CoRP-seq to assess RNA G4 prevalence in human cell transcriptomes.

Main Results:

  • Colistin (COL) selectively binds to parallel G-quadruplexes (G4s).
  • This specific interaction induces G4/COL complex aggregation, allowing for separation via centrifugation.
  • The CoRP-seq protocol demonstrated ultraspecificity, simplicity, and convenience for mapping RNA G4s.

Conclusions:

  • The discovery of COL's specific G4 interaction provides a novel tool for G4 targeting.
  • CoRP-seq offers a straightforward and highly specific method for RNA G-quadruplex profiling.
  • This advancement addresses unmet needs in G4 mapping and the broader field of G4omics.