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Updated: May 5, 2026
10:16
Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
Published on: October 8, 2015
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Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based
Hui Tang1, Dan Guo2,3, Bin Yang4,5
1Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China. huitang0905@zju.edu.cn.
BMC Cancer
|March 6, 2025
Summary
Fine-Needle Aspiration (FNA) aids papillary thyroid carcinoma (PTC) diagnosis. This study links BRAF V600E mutations and right-sided nodules, finding larger tumors and younger patients with the mutation.
Area of Science:
- Endocrinology
- Oncology
- Pathology
Background:
- Fine-Needle Aspiration (FNA) is standard for papillary thyroid carcinoma (PTC) diagnosis, enabling simultaneous cytology and genetic testing.
- The BRAF V600E mutation is highly specific and sensitive for PTC, but its correlation with imaging characteristics via FNA is understudied.
Purpose of the Study:
- To investigate the relationship between BRAF V600E genotype and ultrasound (US) imaging features (location, diameter) in a large cohort of thyroid nodules.
- To explore potential associations between nodule laterality, BRAF V600E status, and lymph node metastasis (LNM) in PTC.
Main Methods:
- Retrospective analysis of 4808 patients undergoing FNA for thyroid nodules.
- Molecular testing for BRAF V600E mutation performed on 2430 patients.
- Correlation of FNA, BRAF V600E status, US findings (location, diameter), and LNM.
Main Results:
- Thyroid nodules were predominantly right-sided (p=0.0004), with a higher prevalence in Bethesda VI cytology (p=0.0041).
- Right-sided PTC showed a higher incidence of ipsilateral LNM (p=0.0007).
- BRAF V600E mutated nodules and right-lobe nodules were significantly larger (p=0.0156); mutation-positive patients were younger.
Conclusions:
- Nodule location, particularly right-sidedness, is associated with PTC and LNM patterns.
- BRAF V600E mutation correlates with larger nodule size and younger patient age, suggesting distinct tumor characteristics.
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