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Applying 3D cultures and high-throughput technologies to study host-pathogen interactions.

Elaine Cristina Pereira De Martinis1, Virgínia Farias Alves2, Marita Gimenez Pereira1

  • 1Ribeirão Preto School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

Frontiers in Immunology
|March 7, 2025
PubMed
Summary
This summary is machine-generated.

Three-dimensional (3D) cell cultures offer advanced models for studying host-microbe interactions. Integrating these systems with molecular methods can reveal complex relationships within the human microbiome.

Keywords:
high-throughput sequencingmicrobial model communitiesorgan-on-a-chiporganoidsrotating wall vessel (RWV)transposon sequencing (TnSeq)tridimensional cell culture

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Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Human microbiome research has advanced significantly due to innovations in cell culturing and DNA sequencing.
  • Three-dimensional (3D) cell culture models mimic in vivo conditions, offering reliable and reproducible in vitro systems.
  • These models are crucial for cell biology, cancer research, and understanding host-microbe interactions.

Purpose of the Study:

  • To provide an overview of 3D culture techniques for studying host-pathogen interactions.
  • To explore the integration of 3D culture systems with high-throughput molecular methods.
  • To discuss the potential of multi-species models for microbiome research.

Main Methods:

  • Review of established and emerging 3D cell culture techniques (e.g., spheroids, organoids).
  • Integration strategies with high-throughput molecular techniques like genomics, transcriptomics, and proteomics.
  • Development and application of multi-species co-culture systems.

Main Results:

  • 3D cell cultures effectively replicate in vivo host microenvironments for studying microbial pathogenesis.
  • Combined approaches enable detailed analysis of host responses and microbial dynamics.
  • Multi-species models facilitate the study of complex interactions involving commensal, beneficial, and pathogenic microbes.

Conclusions:

  • 3D cell culture systems are powerful tools for advancing human microbiome research.
  • Integration with molecular methods enhances the depth of understanding host-microbe interactions.
  • Future research directions include sophisticated multi-species models for comprehensive microbiome analysis.