Related Experiment Video
Updated: May 23, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Over-expression of AeWRKY2 promotes oleanolic acid and hederagenin accumulation in Aralia elata
Honghao Xu1, Hongfei Liu1, Ye Liu1
1Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
Abstract:
Oleanolic acid (OA) and hederagenin from Aralia elata have important medicinal value due to their anti-cancer and anti-inflammatory activities. Transcription factors (TFs) can affect the production of active substances by regulating the expression of genes in metabolic pathways. In this study, a transcription factor AeWRKY2 was cloned and overexpressed in A. elata. The result of gene expression analysis and high-performance liquid chromatography (HPLC) showed that the key enzyme genes of all transgenic strains analyzed were up-regulated to varying degrees. The contents of oleanolic acid and hederagenin were higher than those of wild type, up to 2.773 mg g-1 DW and 0.325 mg g-1 DW, respectively. After one-day MeJA treatment, the contents of oleanolic acid and hederagenin were further increased to 3.31 mg g-1 and 0.37 mg g-1, respectively.
Related Concept Videos
Cell Signaling in Plants
Adaptations that Reduce Water Loss

