Related Experiment Video
Updated: May 23, 2025
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Published on: April 4, 2018
SLC19A1 Gene Polymorphism; Risk Factor for Preeclampsia
Iram Nasir1, Amena Rahim1, Muhammad Afzal1
1Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan.
Objective:
To determine the genotypic and allelic frequency of SLC19A1 (rs1051296 G>T) polymorphism in pre-eclamptic patients and normal pregnant females, and also the genetic association of SLC19A1 (rs1051296 G>T) polymorphisms with the levels of homocysteine (Hcy) and folate in pre-eclamptic Pakistani population.
Study Design:
A case-control study. Place and Duration of the Study: Department of Biochemistry, Islamic International Medical College, Riphah International University, Rawalpindi, Pakistan, from September 2022 to 2023.
Methodology:
Gene association of SLC19A1 (rs1051296 G>T) polymorphism was studied in pre-eclamptic patients to elucidate its clinical significance. The study included 166 pre-eclamptic patients and 166 normotensive pregnant females as controls. Maternal venous blood samples were obtained between 20th and 40th weeks of gestation and DNA was extracted using the Chelex method. Allele-specific polymerase chain reaction (PCR) was used to analyse the SLC19A1 (rs1051296 G>T) polymorphism. Data were analysed using the Chi-square and Student's t-test.
Results:
A significant association in terms of susceptibility was found between SLC19A1 (rs1051296 G>T), CA (p <0.03), and AA (p <0.001) genotype with preeclampsia (PE). A significant association in terms of protection was found between the SLC19A1 (rs1051296 G>T) CC genotype and PE. Notably, low folic acid along with CA (p <0.05) genotype is a high-risk factor for PE. A prior family history of high blood pressure had not proved any significant association with SLC19A1 (rs1051296 G>T, p <0.6).
Conclusion:
SLC19A1 (rs1051296 G>T) polymorphism is a risk factor for PE in the Pakistani population.
Key Words:
Preeclampsia, Blood pressure, Single nucleotide polymorphism, SLC19A1, Polymerase chain reaction.
More Related Videos
05:31Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles
Published on: January 26, 2024
09:52Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq
Published on: April 19, 2013
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
01:28Human Genetics
The complex relationship between genetics and psychology is observable through common biological components such...
02:26Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
01:33Pleiotropy
01:11Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
01:31Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...