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Related Concept Videos

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Related Experiment Video

Updated: May 23, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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UFMylation Modulates OFIP Stability and Centrosomal Localization.

Mulin Yang1, Zihe Zhao1, Jie Di1

  • 1Department of Genetics and Cell Biology, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.

Journal of Clinical Laboratory Analysis
|March 10, 2025
PubMed
Summary

OFIP protein stability and centrosomal localization are regulated by UFMylation, a process mediated by UFL1. This regulation is cell type-specific, impacting ciliogenesis and potentially linked to developmental disorders.

Keywords:
OFIPUFL1UFMylationcentrosomeprotein stability

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • OFIP (KIAA0753) is a centrosomal protein crucial for ciliogenesis, centriolar duplication, and microtubule stability.
  • Mutations in OFIP are linked to Oral-Facial-Digital Syndrome and Joubert Syndrome.
  • UFMylation is a post-translational modification regulating protein function, stability, and localization.

Purpose of the Study:

  • To investigate the interaction between UFL1 and OFIP.
  • To determine if OFIP is a substrate for UFMylation.
  • To elucidate the functional consequences of OFIP UFMylation on its stability and localization.

Main Methods:

  • Immunoprecipitation and immunoblotting to assess protein interactions and UFMylation.
  • Reverse transcription quantitative PCR (RT-qPCR) for mRNA level analysis.
  • Immunofluorescence microscopy for OFIP localization studies.

Main Results:

  • UFL1 interacts with OFIP in vivo and in vitro.
  • OFIP undergoes UFMylation, promoted by UFL1.
  • OFIP UFMylation decreases protein stability and maintains centrosomal localization, with cell type-specific variations.

Conclusions:

  • OFIP is a novel UFMylation substrate.
  • UFL1-mediated OFIP UFMylation is vital for protein stability and centrosomal localization in HeLa cells.
  • Cell type-specific differences in OFIP UFMylation efficacy highlight functional heterogeneity.