Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coffee By-Products: An Overview of Their Antimicrobial Properties.

Molecules (Basel, Switzerland)·2026
Same author

Alicyclobacillin 24: a class III bacteriocin from <i>Alicyclobacillus acidoterrestris</i> targeting species associated with spoilage of acidic fruit-based products.

Frontiers in microbiology·2026
Same author

Benign Acute Childhood Myositis Before and After COVID-19: A Nine-Year Retrospective Study.

Pediatric reports·2026
Same author

In vivo evaluation of the anti-inflammatory efficacy of liposomes loaded with Echinacea purpurea roots extracts.

Drug delivery and translational research·2026
Same author

A novel temperate phage from <i>Alicyclobacillus</i>: first evidence in this genus of genomic identity to a <i>sigK</i>-integrated prophage.

Microbiology spectrum·2026
Same author

Physiological and biochemical responses of twelve autochthonous grapevine varieties to summer stress in the Douro Demarcated Region.

Frontiers in plant science·2026

Related Experiment Video

Updated: May 23, 2025

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
08:23

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

10.4K

Kidney-Derived ECM Hydrogels as Cell Delivery Devices.

Ana M Rodrigues1,2, Sara Gimondi1,2, Rita Quinteira1,2

  • 13B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho, AvePark, Parque de Ciência e Tecnologia, Rua Ave 1, Edificio 1 (Sede), Barco, Guimarães 4805-694, Portugal.

ACS Applied Materials & Interfaces
|March 10, 2025
PubMed
Summary
This summary is machine-generated.

Decellularized kidney extracellular matrix hydrogels support adipose-derived stem cell differentiation for chronic kidney disease (CKD) therapy. These biomimetic scaffolds enhance cell viability and renal phenotype, offering promising kidney regeneration strategies.

Keywords:
adipose-derived mesenchymal stem cellscollagendecellularized kidneyextracellular matrixhydrogelrenal differentiation

More Related Videos

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

15.9K
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
11:19

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

13.3K

Related Experiment Videos

Last Updated: May 23, 2025

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
08:23

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

10.4K
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
09:43

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

15.9K
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
11:19

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

13.3K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Nephrology

Background:

  • Chronic kidney disease (CKD) is a global health issue with limited therapeutic options.
  • Adipose-derived mesenchymal stem cells (ASCs) show promise for CKD treatment, but delivery and survival remain challenging.
  • Hydrogels offer a solution for stem cell delivery, providing support and bioactive cues for kidney regeneration.

Purpose of the Study:

  • To investigate the potential of decellularized kidney extracellular matrix (dKECM) hydrogels for ASC differentiation.
  • To compare dKECM hydrogels with collagen I hydrogels for supporting kidney-specific cell phenotypes.
  • To evaluate the impact of dKECM hydrogels on encapsulated cell viability, proliferation, and renal phenotype.

Main Methods:

  • Characterization of dKECM hydrogels and comparison with collagen I hydrogels.
  • Encapsulation of human ASCs and HK-2 cells within dKECM hydrogels.
  • Assessment of cell viability, metabolic activity, proliferation, morphology, and renal phenotype.

Main Results:

  • dKECM hydrogels demonstrated superior performance compared to collagen I hydrogels.
  • Encapsulated ASCs and HK-2 cells showed enhanced viability, metabolic activity, and proliferation in dKECM hydrogels.
  • dKECM hydrogels promoted a sustained renal phenotype in encapsulated cells.

Conclusions:

  • dKECM hydrogels are a promising biomimetic platform for ASC delivery and kidney regeneration.
  • These hydrogels support cellular activity and phenotype, offering a viable strategy for CKD therapy.
  • Decellularized matrix-based hydrogels represent a potent approach for advancing CKD treatment.