Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advancing quantitative clinical pharmacology competencies in Francophone Africa through an on-line learning framework.

Journal of pharmacokinetics and pharmacodynamics·2026

Same author

Recommendations of the Polish Zebrafish Society on the use of the zebrafish (<i>Danio rerio</i>) model in biomedical research.

Acta biochimica Polonica·2026

Same author

Differential salivary proteome of nonsyndromic cleft lip and/or palate patients with and without dental caries in comparison with nonaffected individuals.

European journal of orthodontics·2026

Same author

High Nasopharyngeal SARS-CoV-2 Load and Delayed Clearance in Hospitalized Patients With Blood Autoantibodies Neutralizing Type I Interferons.

The Journal of infectious diseases·2026

Same author

Standardised Surface Electromyography During Clenching and Swallowing: Patient Perception and Comparison of Two Protocols.

Journal of oral rehabilitation·2026

Same author

Three-Dimensional Evaluation of Secondary Alveolar Bone Grafting in Patients With Bilateral Cleft Lip and/or Palate: A 2-3-Year Post-Operative Follow-Up.

Orthodontics & craniofacial research·2025

Same journal

Accuracy of Curve of Wilson Levelling and Posterior Tooth Inclination With Vestibular, Lingual Straight Wire Technique and Clear Aligners: A Retrospective Cohort Study.

Orthodontics & craniofacial research·2026

Same journal

Structural Associations Between Palatal Morphology and Upper Airway Dimensions: Genetic and Environmental Contributions From A Twin Study.

Orthodontics & craniofacial research·2026

Same journal

Impact of Simulated Obesity in Animals on RANK/RANKL/OPG Pathway During Orthodontic Tooth Movement.

Orthodontics & craniofacial research·2026

Same journal

Methodological Design for Three-Dimensional Assessment of Maxillary and Mandibular Impacted Canines by Cone-Beam Computed Tomography: A Scoping Review.

Orthodontics & craniofacial research·2026

Same journal

Genetic and Environmental Influences on Dental Arch Shape During Development: A Longitudinal Twin Study.

Orthodontics & craniofacial research·2026

Same journal

Is Smartphone-Based 3D Photogrammetry Suitable for Indirect Facial Analysis in Patients With Dentofacial Deformities? A Trueness and Precision Analysis.

Orthodontics & craniofacial research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

Defective Incisor Development in Smad Interacting Protein 1 (Sip1) Null Mice.

Marie De Laet¹, Julie Bertrand¹, Elisa Vingerhoedt¹

¹Department of Oral Health Sciences-Orthodontics, KU Leuven and Dentistry, University Hospitals Leuven, Leuven, Belgium.

Orthodontics & Craniofacial Research

|March 10, 2025

Summary

This summary is machine-generated.

Conditional Smad Interacting Protein 1 (Sip1) gene mutations in mice cause significant dental and craniofacial malformations. This research illuminates potential mechanisms for human developmental anomalies.

Keywords:

Prx1‐Cre Sip1 mice craniofacial and dental abnormalities neural crest cells

More Related Videos

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Published on: May 10, 2019

Related Experiment Videos

Last Updated: Jul 3, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Published on: May 10, 2019

Area of Science:

Developmental Biology
Genetics
Craniofacial Biology

Background:

Smad Interacting Protein 1 (Sip1) plays a critical role in embryonic development.
Targeted inactivation of Sip1 leads to embryonic lethality, necessitating conditional mutation models.

Purpose of the Study:

To histologically and morphologically characterize dental and craniofacial abnormalities in a novel mouse model with a conditional Sip1 gene mutation.
To investigate the role of Sip1 in craniofacial and dental development.

Main Methods:

Utilized a Prx1-Cre mouse model for tissue-specific inactivation of the Sip1 gene.
Analyzed embryos (14.5-18.5 dpc) and Sip1 null mice (newborn and 5-month-old) using immunohistochemistry (β-catenin, Ki67) and morphological examination.
Compared dentofacial measurements between knockout and wild-type mice using the Mann-Whitney U test.

Main Results:

Alterations in incisor position and shape were observed by 15.5 dpc.
Mutant newborns exhibited broadened calvarial sutures, hypoplastic mandibles, serrated alveolar processes, and shorter incisors.
Adult mutants displayed fused sutures, hypoplastic maxilla, and elongated, curved incisors.

Conclusions:

Sip1 is essential for normal dental and craniofacial development.
Conditional Sip1 mutations result in a spectrum of dental and skull malformations.
This model offers insights into human craniofacial and dental anomalies, potentially aiding diagnosis and therapy.