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pH-Activated NIR fluorescent probe for sensitive mitochondrial viscosity detection.

Manlin Fu1, Qingwen Li1, Xue Chen1

  • 1Institute of Natural Medicine and Health Products, School of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Zhejiang 318000, China. jfhu@tzc.edu.cn.

Organic & Biomolecular Chemistry
|March 10, 2025
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Summary
This summary is machine-generated.

A new near-infrared fluorescent probe, SSN, accurately measures mitochondrial viscosity. This advancement aids in understanding cellular health and developing new medical diagnostics and treatments.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cellular Biology
  • Medical Diagnostics

Background:

  • Mitochondrial viscosity is a key indicator of cellular health and function.
  • Accurate measurement of mitochondrial viscosity is vital for medical diagnostics and therapeutic strategies.
  • Existing methods for viscosity measurement may lack sensitivity or specificity in biological systems.

Purpose of the Study:

  • To develop a novel near-infrared (NIR) fluorescent probe for sensitive and accurate measurement of mitochondrial viscosity.
  • To investigate the probe's performance under physiological conditions and in complex biological environments.
  • To demonstrate the probe's utility in real-time monitoring of cellular and organismal viscosity.

Main Methods:

  • Development of a novel NIR fluorescent probe (SSN) utilizing Excited-State Intramolecular Proton Transfer (ESIPT) and Twisted Intramolecular Charge Transfer (TICT) mechanisms.
  • Incorporation of a thiophene-enhanced HBT structure and a hemicyanine moiety for mitochondrial targeting.
  • Evaluation of probe characteristics including large Stokes shift (240 nm) and viscosity sensitivity.

Main Results:

  • The developed probe SSN exhibits high sensitivity to viscosity changes.
  • SSN demonstrates effective mitochondrial targeting and accurate viscosity detection under physiological conditions.
  • Successful application of SSN in HepG2 cells, zebrafish, and glucose-treated systems for real-time viscosity monitoring.

Conclusions:

  • The novel SSN probe offers a sensitive and accurate method for measuring mitochondrial viscosity.
  • SSN has significant potential for advancing medical diagnostics and understanding cellular dysfunction.
  • This probe enables real-time viscosity monitoring in diverse biological contexts, paving the way for new research avenues.