Related Experiment Video
Updated: May 23, 2025
10:06
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
8.9K
ASVirus: A Comprehensive Knowledgebase for the Viral Alternative Splicing.
Yu-Hong Liu1, Hong-Quan Xu2, Si-Si Zhu1
1Key Laboratory of Elemene Class Anti-cancer Chinese Medicines, School of Pharmacy, Hangzhou Normal University, Hangzhou 311121, China.
Journal of Chemical Information and Modeling
|March 10, 2025
Summary
This study introduces ASVirus, a database detailing virus-associated alternative splicing (AS) events. It aids understanding viral infections and identifying therapeutic targets by analyzing splicing changes and regulatory factors.
Area of Science:
- Virology
- Molecular Biology
- Bioinformatics
Background:
- Viruses cause significant global health burdens through pandemics and epidemics.
- Alternative splicing (AS) critically influences host-pathogen interactions during viral infections.
- Understanding virus-related splicing alterations is vital for disease management.
Purpose of the Study:
- To create a comprehensive database of virus-associated alternative splicing (AS) events.
- To consolidate and present data on AS in viral infections for improved understanding.
- To facilitate the exploration of viral mechanisms and therapeutic target identification.
Main Methods:
- Developed ASVirus, a specialized database for virus-associated AS events.
- Integrated high-confidence, experimentally validated splicing data.
- Constructed a gene-splicing factor interaction network to analyze regulatory mechanisms.
Main Results:
- ASVirus provides a user-friendly interface with detailed AS event information across viral families.
- The database includes data on mis-splicing in host genes induced by viral infections.
- It links AS events to their upstream regulatory factors.
Conclusions:
- ASVirus serves as a valuable resource for studying viral infection mechanisms.
- The database aids in identifying novel therapeutic targets for viral diseases.
- Consolidated AS data enhances understanding of virus-host interactions.
Related Concept Videos
02:18Alternative RNA Splicing
20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
01:32RNA Splicing
55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
00:57Viral Recombination
23.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.2K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:36Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
00:36Viral Mutations
32.1K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.1K