Related Experiment Video
Updated: May 5, 2026

Transmitting Plant Viruses Using Whiteflies
Published on: November 8, 2013
Begomovirus Transmission by the Insect Vector, the Whitefly Bemisia tabaci
Hui Du1, Yu-Meng Wang1, Xiao-Wei Wang2
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Abstract:
Begomoviruses are exclusively transmitted by the whitefly Bemisia tabaci in a persistent circulative manner and pose serious threat to the production of important cash crops worldwide. Here, we describe the protocols to study how the whiteflies transmit begomoviruses, including maintenance of whitefly and species identification, preparation of tomato plants infected with virus, acquisition and transmission of virus by whiteflies, dissection of whitefly organs, analysis of virus quantity in whiteflies, immunofluorescence and fluorescence in situ hybridization (FISH) detection of virus in whitefly tissues, membrane feeding of double-stranded RNA (dsRNA) or antibody and microinjection of dsRNA in whiteflies.
More Related Videos
10:38Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
Published on: February 24, 2014
06:25Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
Published on: February 8, 2020
Related Concept Videos
Symbiosis
Arboviral Encephalitis