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Updated: May 23, 2025

Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Full-Length Sequencing of Circular DNA Viruses Using CIDER-Seq
Syed Shan-E-Ali Zaidi1,2, Victor Golyaev3,4, Devang Mehta4,5
1Plant Genetics Lab, TERRA Research and Teaching Centre, Gembloux Agro BioTech, University of Liège, Gembloux, Belgium.
Abstract:
Full-length viral genome sequencing is key for virus distribution profiling, new virus discovery, and understanding virus populations across different samples. Circular DNA Enrichment Sequencing (CIDER-Seq) allows unbiased enrichment and long-read sequencing of circular DNA viruses. CIDER-Seq produces single-read full-length virus genomes, combining PCR-free enrichment with Single Molecule Real-Time sequencing and de-concatenation algorithm. CIDER-Seq data analysis package, using the DeConcat algorithm, processes PacBio sequencing data into intact circular DNA sequences, generating fully annotated and highly accurate circular DNA virus genome sequences.
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