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Updated: Jul 29, 2026
Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
Published on: June 12, 2010
Identification of Interaction Between Geminivirus and Plant Proteins by Bimolecular Fluorescence Complementation
Min Du1, Xueping Zhou2,3, Xiuling Yang4
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Abstract:
Geminiviruses rely heavily on complicated protein-protein interactions between viral proteins and between virus and host plant proteins to establish successful infection. The bimolecular fluorescence complementation (BiFC) assay is based on the in vivo complementation of nonfluorescent component parts of a fluorescent protein through the interaction of proximity target proteins, each fused to a different part of the fluorescent protein. Here, we describe a protocol for the BiFC-based method for the identification of the interaction between geminivirus and plant proteins, which allows for the visualization of protein-protein interactions and the subcellular localization of protein interaction complexes in living systems.
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