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Engineered CRISPR/Cas Ribonucleoproteins for Enhanced Biosensing and Bioimaging
Linxin Cao1, Zeyuan Wang1, Chunyang Lei1
1State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemial Biology, Hunan University, Changsha, 410082, Hunan, China.
Analytical Chemistry
|March 11, 2025
Summary
Molecular engineering enhances CRISPR-Cas systems for superior biosensing and bioimaging. This review details strategies for engineering CRISPR-Cas ribonucleoproteins (RNPs) to improve molecular detection and cellular imaging capabilities.
Area of Science:
- Biotechnology
- Molecular Biology
- Genetics
Background:
- CRISPR-Cas systems are programmable nucleic acid-targeting tools.
- Natural CRISPR-Cas systems have limitations in analytical performance.
- Molecular engineering is crucial for improving CRISPR-Cas capabilities.
Purpose of the Study:
- To review advancements in engineering CRISPR-Cas ribonucleoproteins (RNPs).
- To enhance functional capabilities for molecular detection and cellular imaging.
- To explore strategies for developing advanced CRISPR-Cas RNPs.
Main Methods:
- Cas protein engineering via mutagenesis and fusion techniques.
- Guide RNA engineering through chemical and structural modifications.
- Evaluation of engineered RNPs in biomarker detection and live-cell monitoring.
Main Results:
- Engineered CRISPR-Cas RNPs show improved sensitivity for biomarker detection.
- Applications include live-cell genomic DNA and RNA monitoring.
- Strategies enhance functional capabilities for advanced biosensing and bioimaging.
Conclusions:
- CRISPR-Cas RNP engineering is vital for advanced biosensing and bioimaging.
- Protein and guide RNA modifications significantly boost performance.
- Ongoing research addresses challenges and explores future developments.
