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Updated: May 12, 2026
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
PmAm, a Broad-Spectrum Powdery Mildew Resistance Source Located on Amblyopyrum muticum Chromosome 6T, Is Transferred
Wenjing Xu1,2,3,4, Pengtao Ma5, Huagang He6
1Crop Research Institute, Shandong Academy of Agricultural Sciences (SAAS), Jinan 250100, Shandong, China.
Abstract:
Powdery mildew (caused by Blumeria graminis f. sp. tritici) limits grain production and reduces grain quality in wheat. Amblyopyrum muticum (2n = 2x = 14, TT genomes), a wild relative of wheat, carries agronomically valuable traits, including resistance to powdery mildew, that have not been transferred to wheat. Here, a powdery mildew-resistant Chinese Spring-Am. muticum amphiploid was backcrossed to a powdery mildew-susceptible wheat cultivar, and homozygous resistant derivatives with broad-spectrum resistance were selected. Fluorescence in situ hybridization analysis identified chromosome substitution and compensating Robertsonian translocation lines involving chromosome 6T from Am. muticum.
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