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Updated: Aug 14, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Identification of glycine spin systems in 1H NMR spectra of proteins using multiple quantum coherences
Abstract:
Double-quantum filtered COSY and triple-quantum filtered COSY techniques have been compared for the tripeptide Gly-Tyr-Gly and for human lysozyme. The insertion of a triple-quantum filter in the COSY experiment leads to dramatic spectral simplification in the fingerprint region of the spectrum and permits the specific identification of glycine spin systems in the complex 1H NMR spectra of proteins. The assignment of these peaks to glycine H alpha can be confirmed using 2D double-quantum correlated spectroscopy.
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