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Updated: Jun 19, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Protocol for isolation, fractionation, and system biology-based profiling of gastrointestinal digested dairy
Shashank M Patil1, Ramith Ramu1, Khang Wen Goh2
1Department of Biotechnology & Bioinformatics, JSS Academy of Higher Education & Research, Mysore, Karnataka 570015, India.
Abstract:
Colostrum and milk from dairy sources consist of whey, casein, and fat, which have notable pharmacological properties due to their proteins and peptides. Here, we present a protocol for isolating, simulating in vitro gastrointestinal digestion, and fractionating colostrum and milk hydrolysates from any dairy source. We also describe steps for nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC-ESI-MS/MS) identification of proteins and peptides and in silico system biology-based profiling of the proteins and peptides present in the hydrolysates.
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