A review in analytical progress for house dust mite allergens

House dust mite (HDM) allergens are one of the most important causes of allergenic diseases in the indoor environment. The World Health Organization (WHO) has defined risk thresholds for Group I HDM allergens as a concentration of 2 and 10 μg/mL in dust for producing asthma risk and polar asthma attacks, respectively. Continuing exposure to high concentrations of HDM allergens greatly increases the risk of developing allergic diseases. Therefore, it's necessary to determine the exposure levels of HDM allergens to estimate the risk. So, various approaches have been developed to directly or indirectly detect HDM allergens in the environment. This paper overviews the developmental progress of HDM allergen detection and introduces the principle of HDM allergen detection methods, including semi-quantitative radioallergosorbent test (RAST), ACAREX test, dot immunobinding assay (DIBA), radioimmunoassay (RIA) which combines the high sensitivity and accuracy, enzyme-linked immunosorbent assay (ELISA) with high accuracy, fluorescent multiple arrays which can simultaneously detect multiple HDM allergens, polymerase chain reaction (PCR), and liquid chromatograph-mass spectrometer (LC-MS) with high sensitivity and accuracy. The paper provides an overall understanding of the development of HDM allergen detection methods and guidance for choosing an appropriate method to detect HDM allergens.