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Related Concept Videos

Multipotency and Niche of Bulge Stem Cell01:06

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A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
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Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestion or Explants Result in

Bettina Cattier1,2,3, Rina Guignard1,2,3, Israël Martel1,2,3

  • 1Centre de Recherche en Organogénèse Expérimentale de l'Université Laval/LOEX, Quebec City, QC G1J 5B3, Canada.

International Journal of Molecular Sciences
|March 13, 2025
PubMed
Summary

This study presents an improved method for isolating hair follicle stem cells using enzymatic digestion. This technique enhances early-stage cell recovery and expansion for regenerative medicine applications.

Keywords:
cell culture techniquehair follicleprimary cell cultureregenerative medicinestem cellstissue-engineered skin

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Area of Science:

  • Dermatology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Hair follicle stem cells are multipotent epithelial cells crucial for skin regeneration.
  • Efficient isolation and culturing of these cells are vital for dermatology and regenerative medicine.

Purpose of the Study:

  • To develop an optimized protocol for isolating enriched bulge-derived epithelial cells from scalp specimens.
  • To produce tissue-engineered skin substitutes using these isolated cells.

Main Methods:

  • Epithelium separation from dermis using thermolysin.
  • Microdissection of the hair follicle bulge region.
  • Enzymatic dissociation for single-cell suspension and comparison with explant and benchmark methods.

Main Results:

  • Enzymatic digestion of microdissected bulges yielded 5.3 times more epithelial cells than explant cultures.
  • The enzymatic method showed faster proliferation compared to the benchmark method.
  • Cultured cells maintained comparable morphology, growth rates, and differentiation potential.

Conclusions:

  • The enzymatic digestion method significantly improves early-stage hair bulge cell recovery and expansion.
  • This technique maintains keratinocyte functionality for tissue engineering applications.
  • Offers an efficient method for hair bulge cell extraction in regenerative medicine.