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Updated: Aug 1, 2026
07:39
SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
Published on: June 28, 2019
Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques
Harris Pratsinis1, Eleni Mavrogonatou1, Sevasti-Kiriaki Zervou2
1Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research "Demokritos", Athens, Greece.
Methods in Molecular Biology (Clifton, N.J.)
|March 14, 2025
Summary
Researchers present a strategy to discover novel senotherapeutics from natural products. This approach aims to find compounds that selectively target senescent cells, offering potential treatments for age-related diseases with reduced toxicity.
Area of Science:
- Gerontology and pharmacology
- Natural product chemistry
- Cellular senescence
Background:
- Senescent cells accumulate with age and contribute to age-associated diseases.
- Current senotherapeutics (senolytics and senomorphics) lack universal efficacy due to context-dependent specificity.
- Developing novel senotherapeutics with enhanced specificity and reduced toxicity is crucial.
Purpose of the Study:
- To outline a comprehensive research strategy for screening natural product collections.
- To identify novel senotherapeutic compounds with improved specificity and safety profiles.
- To facilitate the discovery of targeted therapies for age-related conditions.
Main Methods:
- Systematic extraction of natural product sources.
- Isolation and chemical characterization of bioactive compounds.
- In vitro and in vivo biological evaluation of identified compounds for senotherapeutic potential.
Main Results:
- A detailed workflow for senotherapeutic discovery from natural products is proposed.
- The strategy emphasizes specificity and reduced toxicity in compound selection.
- The presented approach guides the identification of promising senotherapeutic candidates.
Conclusions:
- A structured approach to natural product screening can yield selective senotherapeutics.
- This strategy addresses the limitations of current senotherapeutics.
- Further research using this method could lead to effective treatments for aging and related diseases.
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