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Integrative analysis of serum proteomics and transcriptomics in hepatitis C
Jianqiong Wang1, Andong Xia2, Min Tang1
1Department of Clinical Laboratory, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, No.157 Jinbi Road, Kunming, Yunnan, China.
Virology Journal
|March 14, 2025
Summary
This study reveals key serum protein and gene expression differences in hepatitis C (HCV) infection. Identified biomarkers like EIF4A3 may aid in diagnosing and treating HCV.
Area of Science:
- Molecular biology
- Virology
- Biochemistry
Background:
- Hepatitis C is a viral infection transmitted via blood and mother-to-child routes.
- Understanding serum molecular features is crucial for characterizing Hepatitis C Virus (HCV) infection.
Purpose of the Study:
- To characterize the serum molecular profiles of Hepatitis C Virus (HCV) infection using proteomics and transcriptomics.
- To identify differentially expressed proteins and genes (DEPs/DEGs) associated with HCV infection and chronic HCV.
Main Methods:
- Serum samples from control, previous HCV infection, and chronic HCV groups were analyzed.
- Proteomics (TMT) and transcriptomics (RNA-seq) were employed, followed by bioinformatics analysis.
- RT-qPCR and western blot validated key findings.
Main Results:
- Significant differences in serum proteomes and transcriptomes were observed between groups.
- DEPs included immunoglobulins and exosomal proteins; DEGs were involved in extracellular matrix regulation and immunity.
- Key proteins (HSPA4, HSPD1) and genes (KIF11, CENPE) were identified, with interactions involving EIF4A3, MNAT1, and UBE2D1.
Conclusions:
- Proteins EIF4A3, EIF2B1, MNAT1, SNRNP70, and UBE2D1 are implicated in HCV infection and pathogenesis.
- These molecules show potential as biomarkers for hepatitis C diagnosis and treatment.
